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. 1999 Jul 1;19(13):5409–5419. doi: 10.1523/JNEUROSCI.19-13-05409.1999

Fig. 5.

Fig. 5.

Western blot analyses of DARPP-32 protein in samples derived from whole cultures correlate with the numbers of percentage-positive neurons derived from selected field counts.A, Western blot analysis of DARPP-32 protein in E13-derived sister cultures maintained in Neurobasal/B27 with or without BDNF for 1 and 7 DIV. B, BDNF increases the amount of DARPP-32 protein in E13-, E15-, E18-, and P0-derived cultures after 1 DIV. CP, Control caudate putamen, 5 μg from adult mouse; N, Neurobasal/B27; B, Neurobasal/B27 plus BDNF, 100 ng/ml. C, NT-3 and NT-4/5, alone or in combination with BDNF, increase the amount of DARPP-32 protein in E13-derived cultures to the same extent as does BDNF alone. NFG does not increase DARPP-32 protein in sister cultures. The results with GDNF were somewhat variable, and this experiment is one in which GDNF increased the amount of DARPP-32 protein, but not the percentage of immunostained cells. All cultures included Neurobasal/B27 (NB). B, BDNF; N, NGF;3, NT-3; 4, NT-4/5; G, GDNF; All, BDNF, NT-3, and NT-4/5, each at 25 ng/ml.