Fig. 6.
Irregular spiking of cholinergic cells is not attributable to spontaneous inhibitory inputs in vitro.A, B, No discernible alteration in the firing rate or pattern of this cholinergic cell occurs after blockade of GABAA receptors with BIC (30 μm).C, D, Examination of the ISI histograms (bin width = 10 msec; 2 min sample) generated from data from which the spike trains were taken (control data in C) reveals that BIC (30 μm) produces no obvious effect on the spiking rate or pattern. E, F, Grouped data reveal that blockade of GABAA receptors with BIC (30 μm; n = 4) or SR-95531 (30 μm; n = 4) does not produce a significant alteration in the firing frequency (E; control = 4.13 ± 1.64 Hz; BIC or SR-95531 = 3.35 ± 1.57 Hz; n = 8; p > 0.2) or CV (F; control = 0.237 ± 0.097; BIC or SR-95531 = 0.257 ± 0.150; n = 8;p > 0.2) of cholinergic cells.