Table 2.
IL-6 induction by TNF-α, IL-1β, and TGF-β in the presence of the sIL-6R
| Cell treatment2-a | IL-6 bioactivity (pg/ml) | |
|---|---|---|
| Alone | +sIL-6R (100 ng/ml) | |
| Medium | 21.4 ± 4.42-b | 38.1 ± 13.1 |
| TNF-α (50 ng/ml) | 210.6 ± 61.4 | 1,885.3 ± 116.6c,*** |
| TGF-β1 (10 ng/ml) | 184.4 ± 40.3 | 333.0 ± 157.8N.S. |
| IL-1β (2 ng/ml) | 23,275.6 ± 5,334.4 | 1,000,667.0 ± 25,807.9c,*** |
F2-a
Twenty-four hour incubation period.
F2-b
Mean ± SD of three experiments analyzed in triplicate.
F2-c
p values for cytokine versus cytokine + sIL-6R treated samples: ***p < 0.001. N.S., Not significant.