Skip to main content
. 1999 Jul 1;19(13):5429–5434. doi: 10.1523/JNEUROSCI.19-13-05429.1999

Fig. 1.

Fig. 1.

Impairment of astrocyte induction in neuroepithelial cells by the lack of gp130. A, Absence of gp130 signalings in gp130-deficient cells. Neuroepithelial cells from E14.5 gp130 +/+ and gp130 −/− mice were stimulated with either IL-6 plus sIL-6R or LIF. NP-40 lysates were subjected to immunoprecipitation and subsequent immunoblotting with the antibodies indicated, except for the middle panel in which the lysates were directly analyzed by immunoblotting. B, Loss of astrocyte induction in gp130-deficient cells in vitro. Neuroepithelial cells from E14.5 gp130 +/+ (a–d) or gp130 −/− (e–h) mice were cultured as described in Materials and Methods with medium alone (a, e), or IL-6 plus sIL-6R (b, f), LIF (c,g), or CNTF (d, h) and stained for GFAP by specific antibody. GFAP-positive cells and nuclei are shown in red and blue, respectively, by fluorescent microscopy.