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. 1999 Jan 1;19(1):109–118. doi: 10.1523/JNEUROSCI.19-01-00109.1999

Fig. 1.

Fig. 1.

Identification of a Citron–PSD-95 complex in thalamic tissues. A, Presence of a consensus PDZ-binding tSXV motif at the C terminus of Citron. Alignment with the conventional tSXV motif in NR2A and Kv1.4. B, Western blot analysis of Citron expression in various mouse brain areas. Five micrograms of proteins were loaded in each lane. C, Examination of detergent solubility of Citron and several PSD-enriched proteins, including NR2A/B and PSD-95. D, Examination of the potency of three distinct anti-PSD-95 monoclonal antibodies to immunoprecipitate PSD-95 and PSD-95-associated proteins. Note the superiority of one clone, 6G6–1C9 (6G6), over the other two monoclonals [7E3–1B8 (7E3) and K28/58.8.5 (K28)] under our conditions. E, Identification of Citron in a PSD-95 immune complex prepared from thalamic lysates with the 6G6 monoclonal anti-PSD-95 antibody.