PROT immunoreactivity is found in select regions in the forebrain of the adult rat. A, In the somatosensory cortex (corresponding to level 18 in Swanson), PROT immunoreactivity is found in the cytoplasm of perikarya (arrows) located in layer 5. B, A dense array of PROT-labeled processes is found in layer 1 of the piriform cortex (PIR; level 14 in Swanson). Several perikarya with PROT immunoreactivity are found in layer 2, whereas negligible PROT labeling is found in the lateral olfactory tract (lot). C, In the dorsal quadrant of the caudate putamen nucleus (CP), PROT-immunoreactive processes are found in the neuropil surrounding bundles of white matter. D, No PROT immunoreactivity is observed in the dorsal caudate putamen nucleus after preadsorption of the antibody with a 100 nm concentration of the cognate peptide.cc, Corpus callosum. Scale bar, 100 μm.