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. 1999 Jan 1;19(1):180–192. doi: 10.1523/JNEUROSCI.19-01-00180.1999

Fig. 1.

Fig. 1.

Gs-coupled receptors stimulate AC4 in HEK 293 cells. A, HEK 293 cells stably expressing AC4 were treated with increasing concentrations of isoproterenol.B, HEK 293 cells stably coexpressing AC4 with the Gs-coupled 5-HT7 receptor were treated with increasing concentrations of serotonin. Relative cAMP accumulation was determined as described in Materials and Methods, and endogenous HEK 293 cell cAMP accumulation was subtracted from the cAMP measured in AC4-expressing cells. The data are the means ± SD of triplicate assays.