Top. Immunohistochemical detection of AC2 and AC4 in the mouse dentate gyrus. Mouse brain sections (40 μm) were processed for immunohistochemistry as described in Materials and Methods. A, AC2 labeling (red).B, MAP2 labeling of the same image shown inA (green). C, Merged image of A and B(yellow indicates coincident localization).D, AC4 labeling (red).E, MAP2 labeling of the same image shown inD (green). F, Merged image of D and E(yellow as described in C).C′, F′, Higher magnification images of the boxed areas in C andF, respectively. Scale bar, 50 μm. Boxed areas in A and B correspond to that inC. Boxed areas in D andE correspond to that in F.