Bottom. Immunohistochemical detection of AC2 and AC4 in the CA1 region of mouse hippocampus. Mouse brain sections (40 μm) were processed for immunohistochemistry as described in Materials and Methods. A, AC2 labeling (red). B, MAP2 labeling of the same image shown in A (green).C, Merged image of A and B(yellow indicates coincident localization).D, AC4 labeling (red). E, MAP2 labeling of the same image shown in D(green). F, Merged image ofD and E (yellow as described in C). C′, F′, Higher magnification images of the boxed areas inC and F, respectively. Scale bar, 50 μm. Boxed areas in A and Bcorrespond to that in C. Boxed areas inD and E correspond to that in F.