Fig. 10.

Recovery of syntaxin 13–GFP fluorescent signal after bleaching. Embryonic hippocampal neurons 11 DIV were transfected with syntaxin 13–GFP. Selected areas of the axonal network of transfected cells were bleached, and the recovery of the fluorescent signal was followed over a time course of 5 min. The quantitation of the fluorescent signal is expressed as a percentage of the value before bleaching for untreated cells (○), cells treated with 5 μg/ml nocodazole for 30 min (●), and cells treated with 10 nmtaxol for 30 min (▿). The data are the means ± SEM of four independent experiments for untreated and nocodazole-treated cells and the means ± SEM of eight independent experiments for taxol-treated cells.