Fig. 4.

Syntaxin 13–GFP displays a subcellular localization equivalent to that of the endogenous protein.A–C, Embryonic hippocampal neurons 11 DIV transfected with syntaxin 13–GFP were fixed and stained with antibodies against TfR (A) and were imaged to detect syntaxin 13–GFP (B); C shows the merge of the two images, with areas of overlap in yellow.D–I, Hippocampal neurons expressing syntaxin 13–GFP were loaded with 80 μg/ml TxR–Tf for 30 min and either fixed immediately (D–F) or chased for 45 min in medium without labeled Tf before fixation (G–I). Then the cultures were imaged to detect syntaxin 13–GFP (E, H) and TxR–Tf (D, G); F andI show the respective merged images. Scale bars, 5 μm.