Fig. 2.

ONOO⁻ nitrates tyrosine residues in TH and causes the formation of dityrosines.A, TH was treated with the indicated concentrations of ONOO⁻ for 15 min at 30°C and exposed to SDS-PAGE and immunoblotting with a monoclonal antibody specific for nitrotyrosine (diluted 1:1000). B, Blots were stripped and reprobed with a monoclonal antibody specific for TH (diluted 1:20,000). Immunoreactive proteins were visualized by enhanced chemiluminescence with the use of goat anti-mouse secondary antibodies conjugated to horseradish peroxidase. The positions of MW standards (in kilodaltons) are illustrated to the right of the blot.