Neuroepithelial cells express both CNTF and PDGF receptors. One day after plating on glass coverslips, E14 cultures were fixed with 4% paraformaldehyde and then immunostained with antibodies to nestin, CNTF receptor, or PDGF receptor. Secondary antibodies conjugated to AMCA, Cy2, and Cy3, respectively, were used to visualize the target molecules.