Fig. 1.

Hsp40 and Hsp70 chaperones localize to intranuclear inclusions formed by mutant ataxin-3. Shown are immunofluorescence images of representative transfected PC12 neural cells containing intranuclear aggregates formed by truncated, HA-tagged ataxin-3 (HA-Q78) (A) or full-length, myc-tagged NLS-ataxin-3 (Q78) (B). Fixed cells were colabeled with anti-HA or anti-myc antisera to label ataxin-3 (green, left panels) and the indicated anti-Hsp antisera to label the chaperones Hsc70, Hsp70, HDJ-1, or HDJ-2 (red, middle panels). All four chaperones localize to polygln aggregates, as indicated by the merged images (right panels). DAPI staining of nuclei (blue) is included in the left andright panels of A andB.