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Characterization of ET receptor subtypes in pituitary cells. A, Displacement of125I–ET-1 by unlabeled ET-1 and ET-3. Thedottedlines indicate the IC50 values for these two ligands, derived from fitted logistic curves. The results shown are the means from a triplicate incubation. B, Effects of BQ-610 (1 μm) and BQ-788 (1 μm), the ETA and ETB receptor antagonists, respectively, on the ET-1-induced (10 nm) [Ca2+]i response in silent somatotrophs.
