Fig. 9.

Induction of c-fos in cerebellar granule neurons (CGN) by incubation with IL-16. CGN culture medium was changed and replaced with medium containing no IL-16 (−) or 0.1 μm recombinant mouse IL-16 (+). A culture not subjected to medium change (untreated) served as a control for basal levels of Fos in CGN cultures before the medium change. Cells were lysed at various times after the start of exposure to IL-16, as indicated above the lanes. Levels of Fos protein expression were determined by Western analysis. The position of Fos in the gel is indicated on the left.