Fig. 9.

Colchicine blocks the neurotrophin-induced nuclear response. The cell bodies and neurites of DRG neurons were pretreated with 125 μm colchicine or DMSO control for 60 min. Neurons were selectively stimulated at the neurites (A) or at the cell bodies (B) with neurotrophin (+NT) or 0.1 ng/ml BSA control (−NT) after global colchicine treatment. DRG cell body lysates were separated on 10%SDS-PAGE and immunoblotted using anti-pTrk and anti-pCREB. Diagram (top) is a schematic representation of neurons; black bar indicates presence of the Teflon divider. Area of DRG neurons analyzed is indicated by dashed lines.