Fig. 4.

DAT does not colocalize with large dense-core vesicles in DAT-PC12 cells. A, B, Large dense-core vesicles fractionation. [³H]DA-labeled DAT-PC12 cells were fractionated in 0.3–1.2 m sucrose velocity gradients, (A) followed by fractionation of peak [³H]DA-containing fractions on 0.6–1.6m sucrose equilibrium gradients (B).Top, Fractions were analyzed for DAT content by immunoblot. Bottom, Fractionation profile of DAT signal (filled circles) and [³H]DA (in counts per minute; open squares). A representative experiment of two performed is shown.