Table 1.

Mass spectrometry and peptide microsequence analysis of PSD58/53

p58 and p53 tryptic fragments		Predicted IRSp53 tryptic fragments1-a		Peptide microsequence1-d
p58 masses	p53 masses	IRSp53 masses1-b	Position1-c
912.7		913.1	51–58	GYFDALVK
1788.2	1789.7	1788.0	157–171	YSDKELQYIDAISNK
1208.1	1208.4	1208.2	412–421	DGWHYGESEK

^F1-aThe calculated masses of the IRSp53 tryptic fragments that correspond to p58 or p53 masses are listed.

^F1-bAll masses are in daltons.

^F1-cThe amino acid positions of these fragments are based on the numbering system of Yeh et al (1996).

^F1-dPeptide microsequences obtained from the three p58 tryptic fragments were identical to deduced IRSp53 amino acid sequences.