Fig. 3.

Wt-Myc3 expressed in COS7 cells becomes partially resistant to EndoH digestion, but TrJ-Myc3 does not. The wt-Myc3 and TrJ-Myc3 proteins were tagged in loop 3 and expressed in transiently transfected COS7 cells. Cell extracts were prepared 36 hr after transfection, incubated with PNGaseF or EndoH, and separated on 12.5% SDS gels. Untreated extracts were used as a control (−). After blotting, the membranes were incubated with a polyclonal antibody against the c-Myc epitope. The arrow is derived from molecular weight markers and indicates the position of glycosylated (22 kDa) PMP22.