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Synaptoporin and the synaptoporin–synaptobrevin complex in embryonic and adult synaptosomes. Triton X-100 extracts of whole brain crude synaptosomal fractions during different stages of development were immunoprecipitated using the monoclonal antibody against synaptobrevin. Immunoprecipitates (IP) and their corresponding supernatants (S) were analyzed using an antiserum against the synaptophysin analog synaptoporin.
