Fig. 6.

Immunoisolation of synaptic vesicles from embryonic and adult whole brain. Eupergit beads coated with either an antibody against synaptophysin or glycine as negative control were incubated with crude synaptic vesicle fractions as described in Materials and Methods. Membranes adsorbed to the beads, as well as the corresponding supernatants, were analyzed using antibodies against synaptobrevin, synaptophysin, or the α subunit of the Kv1.6 channel. Only beads coated with the antibody against synaptophysin pelleted synaptobrevin and synaptophysin from both embryonic and adult vesicles. The potassium channel protein was always detected in the supernatant.