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Binding of recombinant synaptobrevin constructs to embryonic and adult synaptic vesicles. Crude synaptic vesicle extracts from embryonic or adult brain were incubated with either full-length synaptobrevin (1–116) or the N-terminal part of synaptobrevin (1–96) (see Materials and Methods). The bead pellets and the corresponding supernatants were analyzed using antibodies against synaptophysin, SNAP 25, and syntaxin.
