Fig. 1.

Positional cloning of osm-10.Top, The osm-10 gene maps betweenmec-14 and lin-39 on chromosome III. The osmotic avoidance defect of osm-10(n1602) was rescued by the cosmid T20H4 and the plasmid pKP#51 that contains theosm-10 gene T20H4.1. See Results for details.Bottom, The sequence of the osm-10 cDNA was determined by RT–PCR, leading to the predicted protein sequence shown. The n1602 mutation corresponds to the missense mutation E199K; E199 is lowercase. Amino acids translated in the predicted osm-10(nr2076) protein are in the box. Potential S/T kinase and Y kinase sites are indicated as follows (number of sites): protein kinase C (17) sites areunderlined; casein kinase II (11) sites are inbold; cAMP- and cGMP-dependent kinase phosphorylation sites (6) are in italics; and cyclin-dependent kinase (5) sites are outlined.