Fig. 8.

Immunoblot analysis of the expression and glycosylation of apNEP in different A. californicatissues. A, Twenty micrograms of solubilized membrane proteins (salivary gland, heart, gill, kidney, and CNS) and 30 μg of total protein extracts (buccal ganglion and bag cells) were separated on a 6% SDS-polyacrylamide gel under reducing conditions, blotted, and detected with an anti-apNEP antisera. B, Plasma membrane protein extracts isolated from Aplysia tissues (kidney, CNS) or from transiently transfected mammalian HEK293 cells (HEK293) were incubated in the absence (−) or presence (F) of PNGase F, before loading on the gel.C, SDS-PAGE under nonreducing conditions. Thearrow indicates the position of the 200 kDa band.