Table 1.

Comparison of the action of peptidase inhibitors on apNEP activity present in Aplysia californica CNS and kidney membranes

Inhibitors	Concentration (μm)	% Inhibition (head ganglia membranes)	% Inhibition (kidney membranes)1-a
RB104	1	87	91
HACBO-Gly	10	91	74
Thiorphan	10	41	33
Phosphoramidon	10	41	45
1-10 Phenantroline	5	100	100
Captopril	10	11	0
PMSF	100	0	0

Plasma membranes from head ganglia or kidney [results from Bawab et al. (1993)] were incubated, before the addition of the [³H]Leu-enkephalin, in the absence or presence of various peptidase inhibitors. The enzymatic assays were performed as indicated in Materials and Methods. Results are expressed as a percentage of inhibition of substrate degradation. The percentage inhibition was calculated by comparing the radioactivity under the Tyr-Gly-Gly peak in the presence and absence of inhibitors.

^F1-aBawab et al. (1993).