Table 2.

Comparison of the NEP-like enzymes in the CNS and kidney ofAplysia californica

Characteristics	CNS	Kidney2-a
Activity2-b	1.6 pmol/mg protein per minute	3.5 pmol/mg protein per minute
RB104-KD2-c	0.16 × 10−10	0.26 × 10−10
RB104-Bmax2-e	12 fmol/mg protein	20 fmol/mg protein
RB104-inhibitor gel
electrophoresis (M.W.)	100 and 200 kDa	140 kDa
Western blot (M.W.)2-d	100 and 200 kDa	140 kDa
Northern blot2-e	+f	++

^F2-aBawab et al. (1993).

^F2-bMembrane preperations were incubated with [³H][Leu]enkephalin, in the presence of amastatin at a concentration of 10 μm. The metabolites were separated by RP-HPLC, and the number of counts per minute under the Tyr-Gly-Gly peak were measured as described in Bawab et al. (1993).

^F2-cKidney plasma membranes were incubated in the presence of [¹²⁵I]RB104 and different dilutions of cold I-RB104. The K_D andB_max values were calculated from Scatchard analysis as reported previously (Bawab et al., 1993).

^F2-dWestern blot realized with specific apNEP antibodies.

^F2-eNorthern blot realized with an apNEP cDNA probe.

^fIntensity of the hybridization signal.