Table 1.
Characterization of the vz and cp dissociates by immunocytochemistry
Region | % Total nestin+ | % Total TUJ1+ | % Nestin+ only | % TUJ1+ only | % Both |
---|---|---|---|---|---|
vz | 76.7 | 55.7 ± 2.4 | 45.0 ± 5.2 | 24.0 | 31.7 |
cp | 27.6 | 82.7 ± 5.0 | 17.3 ± 4.1 | 72.4 | 10.3 |
After dissociation, the two populations were double labeled with anti-nestin and TUJ1 antibodies. Approximately half of vz cells express nestin only (45.0%), indicating that they are precursor cells. Neurons identified by TUJ1 staining comprise 55.7% of the vz population. The majority of cp cells were neurons (82.7%), and only 17.3% of the cp cells stained for nestin only. Values are means ± SEM;n = 7.