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. 1999 Jun 1;19(11):4508–4519. doi: 10.1523/JNEUROSCI.19-11-04508.1999

Fig. 4.

Fig. 4.

Preseizure x-ray treatment attenuates cell proliferation and neurogenesis after status epilepticus.A, BrdU labeling and immunohistochemistry in a control adult rat 5 d after saline administration and 6 d after sham irradiation revealed clusters of BrdU-labeled cells in the dentate SGZ.B, C, BrdU immunostaining 5 d after pilocarpine treatment in adult rats that received a single dose of 5 Gy x-irradiation (B) or sham irradiation (C) 1 d before SE is shown. In the sham-irradiated controls, pilocarpine treatment produced a marked increase in dentate SGZ BrdU labeling (C) compared with that in saline treatment (A). This increase was significantly attenuated by a single radiation dose 1 d before SE (B). D, Quantification of BrdU labeling in animals treated as described inA–C showed significantly decreased BrdU labeling in the sham x-rays and saline controls and in the x-rays and SE group compared with the sham x-rays and SE group (mean ± SEM;single asterisk denotes p = 0.002;double asterisks denote p = 0.006). BrdU was given twice, 6 hr apart, on day 4 after pilocarpine or saline treatment for all groups. E, F, TOAD-64 immunostaining 5 d after SE in the same animals analyzed inB and C was similarly decreased by radiation treatment (E) compared with sham irradiation (F). Scale bars:A–C, 100 μm; E,F, 50 μm. dgc, Dentate granule cell layer; h, hilus; m, molecular layer.