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Effect of collapsin-1 on growth cones from inferior olivary nuclei (A, B,OLIVE) and basilar pontine nuclei (C,D, PONS). Most olivary growth cones maintained their lamellipodia and continued to advance after exposure to collapsin-1 (∼50 ng/ml) for 20 min (compare A andB). Most pontine growth cones collapsed within 20 min after collapsin-1 exposure (∼20 ng/ml) and became neurites with tapered endings that did not extend further or retracted slightly (compare C and D). Scale bar, 20 μm.
