Fig. 8.

Southern blot of products obtained by RT-PCR of mRNA from the utricular macula (lanes 1–3), retina (lanes 4–6), kidney (lanes 8–10), and gut (lanes 11–13) using primers designed to amplify a 1.4 kbp product comprising the entire intracellular domain and the membrane proximal region of the SCA. Positions of markers are indicated in kilobase pairs. Lanes 1, 4, 8, and 11 are controls without RNA;lanes 2, 5, 9, and 12 are controls without RT; and lanes 3, 6, 10, and 13are the products of complete RT-PCR reactions. Size markers were run inlane 7.