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CAP from C57BL/6N sciatic nerves at 48 hr incubation in Optimem I medium. Nerves were incubated for 3–24 hr at 37°C before being cooled to 25°C. The CAP values at time 0, 3, and 24 hr at 37°C are shown for comparison to demonstratethe decrease in CAP size in the first 3 hr after removal of the nerve to culture. A, A wave. B, C wave. Complete preservation of both A and C waves is seen only if the cooling occurs by 12 hr after axotomy. *p < 0.05.
