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. 1999 Jun 15;19(12):4705–4717. doi: 10.1523/JNEUROSCI.19-12-04705.1999

Fig. 7.

Fig. 7.

Current–voltage (I–V) relationships for wild-type rSERT and D98E mutant conducting states. Currents were measured in a single voltage-clamped (−40 mV) oocyte during voltage-ramp protocols (−120 to +60 mV in 10 sec) in the presence of either buffer (control,Ctrl), 5HT 5 μm, or the transporter antagonist citalopram 30 μm (Cit).A, I–V plots for currents for wild-type rSERT. B, I–V plots for currents for D98E mutant. All experiments were performed at pH 5.0. Data shown are representative of recordings from single oocytes expressing either wild-type rSERT or D98E mutant (n = 9–12).C, Effect of pH on reversal potential of substrate-independent transporter-associated leak current (ICtrl–ICit) for rSERT (■, solid line) and D98E mutant (▪,dashed line). Data plotted are means ± SEM (n = 7–9) fit to least- square linear regression with slopes of −42 mV/pH U (rSERT;r = 0.99) and −46 mV/pH U (D98E;r = 0.99). D, Effect of pH on reversal potential of 5HT-activated transporter-specific current (I5HT–ICit) for rSERT (■, solid line) and D98E mutant (▪,dashed line). Data plotted are means ± SEM (n = 7–12) fit to least-square linear regression with slopes of −28 mV/pH U (rSERT;r = 0.99) and −53 mV/pH U (D98E;r = 0.98).