Table 1.

Pharmacological characterization of wild-type rat SERT and D98E mutant

Drug	5-HT uptake Ki or Km (nm unless otherwise indicated)
	rSERT	D98E
Paroxetine	0.3  ± 0.09	0.5  ± 0.14
Mazindol	272  ± 63	231  ± 62
Cocaine	390  ± 50	1490  ± 601-a
Imipramine	38  ± 8	190  ± 70
Citalopram	2.2  ± 0.5	270  ± 901-a
5-HT1-b	460  ± 210	400  ± 160
Na+	19.9  ± 2.3 mm	>100 mm1-a
Cl−	5.2  ± 1.6 mm	41.3  ± 2.5 mm

K_i and K_m values for various compounds for [³H]5HT uptake in HeLa cells transiently transfected with wild-type rSERT or the D98E mutant. [³H]5HT uptake experiments were performed as described in Materials and Methods. Data represent means ± SEs from results of three experiments performed in triplicate.

^F1-ap < 0.05, compared with rSERT value.

^F1-bV_max values (mol/min per cell) for 5-HT transport determined in COS-7 cells were rSERT, 5.0 ± 2.4 × 10⁻¹⁸ and D98E, 2.4 ± 1.2 × 10⁻¹⁸.