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. 1999 May 15;19(10):3791–3800. doi: 10.1523/JNEUROSCI.19-10-03791.1999

Fig. 2.

Fig. 2.

Dark-field photomicrographs of in situ hybridization preparations for IL-6 (A,C, E, G) or GAP-43 (B, D, F,H) mRNA, all of sections of L5 DRG. DRG are contralateral (A, B) or ipsilateral (C, D) to sciatic nerve transection, ipsilateral to intraneural injection of colchicine (E,F), or ipsilateral to nerve transection plus injection of colchicine into the nerve stump (G,H). Note that IL-6 mRNA is induced by nerve transection but not by intraneural injection of colchicine and that the effect of nerve transection on IL-6 mRNA is blocked by more proximal injection of colchicine. GAP-43 mRNA is also induced by nerve transection. However, in contrast to IL-6 mRNA, GAP-43 mRNA is induced by intraneural injection of colchicine, and its induction by nerve transection is not blocked by more proximal injection of colchicine. Despite longer exposure times, the signal for IL-6 mRNA is consistently weaker than that for GAP-43 mRNA, presumably because the latter is much more abundant. Magnification, 180×.