Table 8.
Time-dependent redistribution of microtubules in collaterals treated first with nocodazole and then vinblastine
| Time after treatment (min) | % of collaterals containing microtubules (length in μm) | |||
|---|---|---|---|---|
| <10 | 11–20 | 20–30 | >30 | |
| No treatment | 15 (236) | 54 (111) | 76 (26) | 93 (69) |
| NOC only | ||||
| 3 | 3 (118) | 35 (24) | 72 (23) | 85 (20) |
| 6 | 1 (98) | 34 (29) | 60 (15) | 78 (14) |
| 9 | 0 (113) | 10 (42) | 63 (11) | 94 (18) |
| 15 | 0 (147) | 2 (54) | 6 (16) | 57 (19) |
| NOC then X | ||||
| DMSO | ||||
| 15 | 16 (145) | 48 (74) | 60 (20) | ND |
| 30 | 14 (211) | 41 (44) | 87 (15) | ND |
| 60 | 15 (543) | 66 (116) | 92 (34) | ND |
| Vinblastine (8 nm) | ||||
| 15 | 3 (248) | 19 (101) | 39 (61) | ND |
| 60 | 8 (210) | 40 (65) | 48 (21) | ND |
| Vinblastine (20 nm) | ||||
| 15 | 1 (203) | 4 (48) | 33 (18) | ND |
| 30 | 3 (201) | 7 (58) | 21 (24) | ND |
| 60 | 2 (216) | 11 (111) | 53 (17) | ND |
Cultures were treated with 2 μg/ml nocodazole (NOC). Data were collected from three separate experiments in each experimental condition. X, 15 min after NOC treatment; ND, not determined. As a baseline comparison point for the data on the effects of nocodazole and the time course of microtubule reinvasion, data from untreated cultures are also presented (same data as in Table 3).