Fig. 4.

Developmental expression of DSD-1-PG in mouse brain lysates. Total protein (100 μg) extracted from brains of the indicated ages (extraction buffer: 50 mm Tris, pH 8, 50 mm sodium acetate, 60 mmn-octyl-β-d-glucopyranoside) were resolved on 4–10% SDS-PAGE gels and blotted onto polyvinylidene difluoride (PVDF) membrane. Extracts were either undigested (−) or predigested (+) with chondroitinase ABC (ChABC) as shown. Blota was developed with 473HD mAb, and blotb was developed with the anti-DSD-1-PG polyclonal serum KAF13.