Skip to main content
. 1999 Oct 15;19(20):8866–8875. doi: 10.1523/JNEUROSCI.19-20-08866.1999

Fig. 3.

Fig. 3.

Enrichment of PrPC in the synaptic plasma membrane fraction but not in the postsynaptic density fraction isolated from mouse brains. Equal amounts (100 μg/lane) of subcellular fractions from wild-type mouse brain and prion protein-deficient mouse brain (Prnp0/0) were analyzed by immunoblotting with anti-PrP antibody 3B5 (A) and antiserum Ra5 (B). For comparison, subcellular fractions of PrPC-overexpressing tg 35 mice (30 μg/lane) were also examined using antibody Ra5 (C). The immunostaining for synaptotagmin (D) and NMDA receptor subunit NMDA-R1 (E) was used as a control for synaptic vesicle proteins and postsynaptic membrane proteins, respectively. Subcellular fractions are designated as follows: H, homogenate; SV, crude synaptic vesicle fraction; CS, cytosolic synaptosomal fraction; SPM, synaptic plasma membranes.F, In the PSD fraction, PrPC is not detectable (Western blot analysis in homogenates and PSD with antibody 3B5; 30 μg of protein were loaded in each lane). G, Immunostaining for NMDA-R1 was used as a control for postsynaptic membrane proteins.