Fig. 3.

Absence of any immediate AChR modulation by PACAP. α7- and α3*-AChR responses (black and gray bars, respectively) to 20 μm nicotine coapplied with 100 nm PACAP38 (Nic + PACAP) are compiled relative to responses from neurons tested in parallel that were challenged with 20 μm nicotine alone (dashed line). Under such conditions, PACAP38 failed to detectably alter the AChR responses (p > 0.1 for each bar) either when AC was left intact (−) or after AC inhibition (+) by 10 min pretreatment with 200 μm ddA (n = 7–10 neurons tested with Nic + PACAP or with Nic alone for each condition).