Fig. 5.

PACAP mobilizes intracellular Ca²⁺ stores. A, Application of PACAP38 elevates [Ca²⁺]_i in both ddA-dialyzed and intact ciliary ganglion neurons. A1, Image obtained using bright-field optics that depicts a field of three neurons bathed in normal recording solution. A whole-cell recording has been established on the center neuron, causing its intracellular contents to be dialyzed with pipette solution containing 200 μm ddA to inhibit AC. A2,A3, The same field as in A1 showing images obtained using epifluorescence optics taken 0 and 90 sec after initiating bath perfusion with normal recording solution containing 100 nm PACAP38. Note the increased Fluo-3AM fluorescence in each of the three neurons. B, PACAP38 elevates [Ca²⁺]_i in ciliary ganglion neurons bathed in recording solution lacking added Ca²⁺.B1, Bright-field optics. B2,B3, Epifluorescence images taken 0 and 90 sec after initiating perfusion with 0 Ca²⁺ recording solution containing 100 nm PACAP38. Scale bar (shown inB3 for all panels): 20 μm.