Table 1.
Peptides from this study and their measured NMR parameters
| Sequencea | INN/IαNF4–L51-b | Δδ pH F4 (ppm)1-c |
|---|---|---|
| PFLRFad | 0.0e | NAe |
| DPFLRFad | 0.20f | 0.128f |
| SDPFLRFa | 0.48 (4°C) | 0.33 |
| 0.51 (15°C) | ND | |
| 1.58 (26°C) | ND | |
| GDPFLRFa | 0.45 (4°C) | 0.31 |
| 0.72 (15°C) | ND | |
| 1.54 (26°C) | ND | |
| NDPFLRFa | 0.24 | 0.33 |
| pQDPFLRFa | 0.28 | 0.35 |
| GYPFLRFad | 0.0 | NA |
| pQYPFLRFad | 0.0 | NA |
NA, Not applicable (no titrating group); ND, not done.
F1-a
One-letter amino acid code with “pQ” = pyroglutamic acid and with “a” = NH2.
F1-b
Ratio of intensities of amide-to-amide (INN) and α-to-amide (IαN) ROESY cross peaks between F4 and L5.
F1-c
Magnitude of the shift (Δ) in chemical shift (δ) of the amide proton of F4 as a function of pH. The values represent the shift at highest pH minus the shift at lowest pH.
dNot naturally occurring.
F1-e
Data are for F2 and L3.
fData are for F3 and L4.