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. 1999 Aug 1;19(15):6360–6371. doi: 10.1523/JNEUROSCI.19-15-06360.1999

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Copyright © 1999 Society for Neuroscience

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Fig. 6. — Differential sedimentation of^(FLAG)β subunits on sucrose density gradients. COS cells transfected with ^(FLAG)β3,^(FLAG)β3^(DNTK), or^(FLAG)β2^(GKER) were subjected to sucrose density gradient fractionation 16 hr after transfection. Gradient fractions were separated by SDS-PAGE; the^(FLAG)β subunits were detected by Western blotting using anti-FLAG M2 monoclonal antibody (A), and the signals were quantified using a Bio-Rad phoshorimager (B, ■, ^(FLAG)β3, ●,^(FLAG)β3^(DNTK); C, ○,^(FLAG)β2, ♦,^(FLAG)β2^(GKER)). The data for^(FLAG)β2 are taken from Gorrie et al. (1997) and represent immunoprecipitation of this protein from expressing cells after metabolic labeling with [³⁵S]methionine. The level of β2 in each fraction was quantified using a Bio-Rad phosphorimager. Sedimentation coefficients of receptor subunits were determined by reference to the standards BSA (4.3S), aldolase (7.4S), and catalase (11.2S).