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Mitotracker and cytochrome cstaining
| Mitotracker+cells | Mitotracker− cells | ||
|---|---|---|---|
| Cytc+ | Cyt c− | ||
| Control | 84 | 0 | 16 |
| Campto | 31 | 4 | 65 |
| Campto + Flavo | 86 | 0 | 14 |
| Campto + BAF | 33 | 35 | 32 |
E18 cortical neurons were treated with medium alone (Control) or 10 μm camptothecin (Campto) alone or in combination with 1 μm flavopiridol (Flavo) or 100 μm BAF. Ten hours later, the cells were assessed for Mitotracker and cytochromec (Cyt c) staining, as described in Materials and Methods.
