Fig. 1.
Cytoprotective effects of SNAP on the survival of PC12 cells in serum-free conditions. Cells were collected, washed extensively with serum-free RPMI 1640 medium, and replated onto collagen-coated 24-well plates at a density of 2.5 × 105 cells per well in a volume of 1 ml of serum-free media. Cells were treated with the same concentration of the NO donor SNAP. At 24 hr, cell viability was determined by counting intact nuclei after lysing of the cell membrane (mean ± SD.n = 3). *p < 0.01 versus nonpretreatment.