Skip to main content
. 1999 Aug 15;19(16):6740–6747. doi: 10.1523/JNEUROSCI.19-16-06740.1999

Fig. 5.

Fig. 5.

SNAP and 8-Br-cGMP prevent mitochondrial cytochrome c release and caspase-3 activation in serum-deprived PC12 cells. Cells were treated with SNAP (100 μm), 8-Br-cGMP (1 mm), ODQ (40 μm), or KT5823 (180 nm) in serum-free media. After 6 hr for cytochrome c release and 12 hr for caspase-3 activation, cells were harvested and homogenized, and then the cytosolic fraction was prepared by centrifugation at 100,000 × g. Cytochrome c release (A) and caspase activation (B) were determined by Western blot analysis.