Fig. 9.

Evidence that amontillado is required for a hatching behavior: larval head swinging.A, The absence of the amontillado region results in a significant reduction in the frequency of head swinging. Stage 17 embryos were videotaped, and the tapes were replayed for analysis. Each head swing is indicated by a vertical line. Hatching is indicated by an arrow. Thehorizontal axis shows increasing time in minutes. The behavioral profiles of three separate individuals from each class are shown, chosen to represent the range of profiles observed. Thetop three graphs are from wild-type Canton S larvae. Themiddle and bottom three graphs are embryos from the cross of Df Tl^9QRXand Df ro^80b. The middle set corresponds to embryos that hatched, namely DfTl^9QRX/TM3Sb or Dfro^80b/TM3Sb. The bottom set corresponds to motile nonhatching larvae carrying one copy of each of the two overlapping deletions Df(3R)Tl^9QRX/Df(3R)ro^80b.B, Expression of wild-type, but not of mutant,amontillado rescues the head-swinging defect. Shown is the distribution of the frequency of swinging behavior in wild-type and mutant embryos with and without amontillado. The number of swings observed within a 3 hr period was counted for 22 heat-shocked Df(3R)Tl^9QRX/Df(3R)ro^80blarvae, 31 heat-shocked Df(3R)Tl^9QRX/Df(3R)ro^80blarvae carrying the hs:amon transgene, and 25 heat-shocked Df(3R)Tl^9QRX/Df(3R)ro^80blarvae carrying the hs:amon^H237Atransgene. Twenty wild-type Canton S larvae were analyzed for 3 hr before hatching. The percentage of the population of each strain whose frequency of swinging fell into each of the bins indicated on thex-axis was plotted for each genotype. The mean (±SE) for each of the four different classes, from front to back, was 75 (± 16), 58 (± 13), 147 (± 17), and 275 (± 21).