Skip to main content
. 1999 Aug 15;19(16):6723–6732. doi: 10.1523/JNEUROSCI.19-16-06723.1999

Fig. 3.

Fig. 3.

TeNT treatment cleaves synaptobrevin/VAMP2 without blocking SV recycling in neurons before synaptogenesis. A, B, Immature control neuron exposed to Syt-ecto Abs (for 5 min in the presence of 55 mm KCl in the external medium) (B) and double labeled for synaptobrevin/VAMP2 (A). C,D, Immature neuron exposed to Syt-ecto Abs (D) and double labeled for synaptobrevin/VAMP2 (C) after treatment with 10 nm TeNT. Note that TeNT treatment cleaves synaptobrevin/VAMP2 without impairing SV recycling. Scale bar, 11.25 μm. E, Quantitative analysis of Syt-ecto Ab internalization (●) and synaptobrevin/VAMP2 cleavage (■) in developing neurons at different times after culture intoxication. Note that TeNT treatment dramatically reduces synaptobrevin/VAMP2 immunoreactivity over time without significantly reducing SV recycling. F, Quantitative analysis of Syt-ecto Ab internalization (●) and synaptobrevin/VAMP2 cleavage (■) in developing neurons exposed to increasing doses of BoNT/F. Note the existence of a strict correlation between synaptobrevin/VAMP2 cleavage and inhibition of SV recycling. Values were expressed as a ratio between the signals produced by Syt-ecto Abs or by synaptobrevin/VAMP2 antibodies and those produced by antibodies directed against total synaptotagmin I or synaptophysin.