Fig. 1.

Peak current density of agonist-evoked responses is larger for recordings from cells transfected with NR1A/NR2A than NR1A/NR2B. A₁,A₂, Representative traces of open tip recording (A₁) or whole-cell current (A₂) made by switching between NMG-containing and NaCl-containing extracellular solutions.B, Representative current traces in response to 20 msec application of 1 mm glutamate (in continuous presence of 50 μm glycine) recorded from a cell transfected with either NR1A/NR2A (thick line) or NR1A/NR2B (thin line). C, NR1A/NR2B current trace (thin line) normalized to the peak of an NR1A/NR2A current trace (thick line) to illustrate difference in activation time courses. D, Bars show mean ± SEM for 10–90% rise-time (n = 10 for both subtypes; **p < 0.01 by unpaired t test).E, Mean ± SEM for 10–90% decay time (n = 10 for both subtypes; **p< 0.01 by unpaired t test). F, Bars indicate mean ± SEM for current responses to 100 μmglutamate (in the continuous presence of 50 μmglycine) recorded from n = 60 (NR1A/NR2A) orn = 106 (NR1A/NR2B) different cells. **p < 0.001 by unpaired ttest.