Fig. 3.
Synaptogenesis was not affected by chronic BDNF treatment. A, Fluorescence image of an autaptic neuron labeled with an antibody directed against synapsin Ia,b and visualized using a Texas Red-conjugated secondary antibody. Each bright puncta represents a presynaptic specialization. Scale bar, 20 μm.B, The number of synapses per neuron did not differ between treatment groups. Average numbers of synapses per cell were (mean ± SE) 506 ± 58 in controls and 594 ± 74 in BDNF-treated neurons (n = 13 and 7, respectively;p > 0.37). C, The average size of synapses was unaffected by BDNF. Mean synapse size, with each synapse defined as the pixel area of an isolated puncta, was 0.35 ± 0.01 and 0.37 ± 0.04 μm2 per synapse for control (n = 13) and +BDNF (n = 7) cells, respectively; p > 0.56.