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Immunoprecipitation of UV cross-linked complexes by anti-Hu ELAV-like antibodies: SDS gel analysis of immunoprecipitated complexes formed between PC12 cell extracts (lane 1), assembled MT preparations isolated from PC12 cells (lane 2), or purified HuD-GST (lane 3) UV cross-linked to [32P]-labeled tau RNA fragment I. NIS is the complex formed with normal serum (lane 4).Lanes 5 and 6 show unprecipitated PC12 extract and GST-HuD protein analyzed immediately after cross-linking.
